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korea plasmid pet 28a e coli expression vector  (Addgene inc)


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    Addgene inc korea plasmid pet 28a e coli expression vector
    Korea Plasmid Pet 28a E Coli Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/korea plasmid pet 28a e coli expression vector/product/Addgene inc
    Average 94 stars, based on 103 article reviews
    korea plasmid pet 28a e coli expression vector - by Bioz Stars, 2026-02
    94/100 stars

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    Target fusion genes and general scheme of the <t>pET-28a(+)-based</t> expression vector for the expression of HBc/G-SARS-CoV-2 fusion proteins. ( A ) NcoI/BamHI fragments used in the cloning (blue boxes represent the partial or full sequence of the HBc/G gene, numerals indicate the length of the HBc/G protein, the RBM fragment is shown in green, the B-cell epitope containing peptide of the N protein is shown in yellow, and the string of five CTL epitopes from the N protein is shown in red. The names of the corresponding expression plasmids are given on the right side. ( B ) General scheme of the pET-28a(+)-based expression vector. Kanamycin resistance gene is shown in orange, HBc/G is shown in blue; inserts: RBM in green, Bep in yellow, CTL string in red.
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    Target fusion genes and general scheme of the <t>pET-28a(+)-based</t> expression vector for the expression of HBc/G-SARS-CoV-2 fusion proteins. ( A ) NcoI/BamHI fragments used in the cloning (blue boxes represent the partial or full sequence of the HBc/G gene, numerals indicate the length of the HBc/G protein, the RBM fragment is shown in green, the B-cell epitope containing peptide of the N protein is shown in yellow, and the string of five CTL epitopes from the N protein is shown in red. The names of the corresponding expression plasmids are given on the right side. ( B ) General scheme of the pET-28a(+)-based expression vector. Kanamycin resistance gene is shown in orange, HBc/G is shown in blue; inserts: RBM in green, Bep in yellow, CTL string in red.
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    Target fusion genes and general scheme of the pET-28a(+)-based expression vector for the expression of HBc/G-SARS-CoV-2 fusion proteins. ( A ) NcoI/BamHI fragments used in the cloning (blue boxes represent the partial or full sequence of the HBc/G gene, numerals indicate the length of the HBc/G protein, the RBM fragment is shown in green, the B-cell epitope containing peptide of the N protein is shown in yellow, and the string of five CTL epitopes from the N protein is shown in red. The names of the corresponding expression plasmids are given on the right side. ( B ) General scheme of the pET-28a(+)-based expression vector. Kanamycin resistance gene is shown in orange, HBc/G is shown in blue; inserts: RBM in green, Bep in yellow, CTL string in red.

    Journal: Vaccines

    Article Title: Toward a SARS-CoV-2 VLP Vaccine: HBc/G as a Carrier for SARS-CoV-2 Spike RBM and Nucleocapsid Protein-Derived Peptides

    doi: 10.3390/vaccines12030267

    Figure Lengend Snippet: Target fusion genes and general scheme of the pET-28a(+)-based expression vector for the expression of HBc/G-SARS-CoV-2 fusion proteins. ( A ) NcoI/BamHI fragments used in the cloning (blue boxes represent the partial or full sequence of the HBc/G gene, numerals indicate the length of the HBc/G protein, the RBM fragment is shown in green, the B-cell epitope containing peptide of the N protein is shown in yellow, and the string of five CTL epitopes from the N protein is shown in red. The names of the corresponding expression plasmids are given on the right side. ( B ) General scheme of the pET-28a(+)-based expression vector. Kanamycin resistance gene is shown in orange, HBc/G is shown in blue; inserts: RBM in green, Bep in yellow, CTL string in red.

    Article Snippet: E. coli expression vectors based on the pET-28a(+) plasmid with the appropriate HBc/G fusion protein gene ( ) were commercially obtained from BioCat GmbH (Heidelberg, Germany).

    Techniques: Expressing, Plasmid Preparation, Cloning, Sequencing